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cd34 positive selection kit  (Miltenyi Biotec)


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    Miltenyi Biotec cd34 positive selection kit
    Cd34 Positive Selection Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+positive+selection+kit/CD34+MicroBead+Kit%2C+human/bio_rxiv__2025__08__31__672613-219-26-29
    Average 97 stars, based on 1642 article reviews
    cd34 positive selection kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Purification:

    Article Title: Low input capture Hi-C (liCHi-C) identifies promoter-enhancer interactions at high-resolution
    Article Snippet: .. Hematopoietic stem and progenitor cells (HSC; CD34 + , CD38 − ) common myeloid progenitors (CMP; CD34 + , CD38 + , CD33 + ) and common lymphoid B-cell progenitors (CLP; CD34 + , CD38 + , CD19 + ) were purified by Miltenyi Biotec CD34-positive selection kit (cat. #130-046-703) and fluorescence-activated cell sorting (FACS) from four donations (two per biological replicate) of 15-to 22-week-old human fetal liver and fetal bone marrow as previously described . .. Briefly, after CD34 + selection, positive cells were stained for flow cytometry with the following fluorophore-conjugated monoclonal antibodies, all from BD Biosciences, and used at the manufacturer’s recommended concentration: CD34 PECy7 (cat. #348811; 1:20 dilution), CD38 FITC (cat. #555459; 1:5 dilution), CD19 BV421 (cat. #562440; 1:20 dilution) and CD33 APC (cat. #551378; 1:5 dilution).

    Selection:

    Article Title: Low input capture Hi-C (liCHi-C) identifies promoter-enhancer interactions at high-resolution
    Article Snippet: .. Hematopoietic stem and progenitor cells (HSC; CD34 + , CD38 − ) common myeloid progenitors (CMP; CD34 + , CD38 + , CD33 + ) and common lymphoid B-cell progenitors (CLP; CD34 + , CD38 + , CD19 + ) were purified by Miltenyi Biotec CD34-positive selection kit (cat. #130-046-703) and fluorescence-activated cell sorting (FACS) from four donations (two per biological replicate) of 15-to 22-week-old human fetal liver and fetal bone marrow as previously described . .. Briefly, after CD34 + selection, positive cells were stained for flow cytometry with the following fluorophore-conjugated monoclonal antibodies, all from BD Biosciences, and used at the manufacturer’s recommended concentration: CD34 PECy7 (cat. #348811; 1:20 dilution), CD38 FITC (cat. #555459; 1:5 dilution), CD19 BV421 (cat. #562440; 1:20 dilution) and CD33 APC (cat. #551378; 1:5 dilution).

    Article Title: Decoding human B cell ontogeny in prenatal and adult bone marrow and in vitro models via single-cell multiomics
    Article Snippet: After centrifugation, red blood cells were lysed by treating with RBC lysis buffer according to manufacturer’s instructions (eBioscience #00-4300). .. Finally, cells were counted and processed in one or more of the following way; (1) sequencing the whole population, (2) enriching for CD34-positive cells using the CD34-positive selection kit (Miltenyi Biotec #130-046-702), following manufacturer’s instructions, and sequencing, (3) enriching for CD34-positive cells and sorting for LMPP and MPP subpopulations . .. Staining antibodies: Lin-FITC: CD2 clone: RPA-2.10 (BioLegend), CD3 clone OKT3 (BioLegend), CD14 clone M5E2 (BioLegend), CD16 clone 3G8 (BioLegend), CD56 clone HCD56 (BioLegend), CD235a clone Ga-R2/HiR2 (BD Biosciences); CD34-BV786 clone 581 (BD Biosciences); CD38-BV711 clone HIT2 (BioLegend); CD90-APC-Cy7 clone 5E10 (BioLegend); CD45RA-APC clone HI100 (BioLegend), and DAPI as live death dye.

    Article Title: The synthetic phospholipid C8-C1P determines pro-angiogenic and pro-reparative features in human macrophages restraining the proinflammatory M1-like phenotype.
    Article Snippet: .. Using the CD34 positive selection kit (Miltenyi Biotec, Bergisch Gladbach, Germany), endothelial colony forming cells (ECFCs) were isolated from umbilical cord blood collected after normal full-term deliveries with the written informed consent of the mother, as described previously (9, 18). ..

    Article Title: DLL4 and VCAM1 enhance the emergence of T cell–competent hematopoietic progenitors from human pluripotent stem cells
    Article Snippet: .. CD34 + cells were enriched using the CD34-positive selection kit (Miltenyi Biotec, 130-046-702) according to the manufacturer’s instructions. .. CD34 + -enriched cells were cryopreserved using CryoStor CS10 (STEMCELL Technologies, 07930) for use in downstream culture.

    Article Title: DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells
    Article Snippet: .. CD34+ cells were enriched using the CD34 positive selection kit (Miltenyi Biotec, 130-046-702) according to the manufacturer’s instructions. .. CD34+ enriched cells were cryopreserved using CryoStor CS10 (StemCell Technologies, 07930) for use in downstream culture.

    Article Title: Time- and lineage-resolved transcriptional profiling uncovers gene expression programs and clonal relationships that underlie human T lineage specification
    Article Snippet: Cells were mixed approximately 30 times to achieve dissociation to a single cell suspension and TrypLE was diluted in HF buffer (Hanks’ balanced salt solution, Thermo Fisher Scientific, 14175103, + 2% FBS, Thermo Fisher Scientific, 12483020) and then cells were spun at 300xg for 5 minutes and washed in HF. .. Next the CD34-positive selection kit (Miltenyi Biotec, 130-046-702) was used to enrich for CD34+ cells which were cryopreserved using CryoStor CS10 (STEMCELL Technologies, 07930) following the manufacturer’s instructions. .. EHT media was prepared by combining complete StemPro34 (Thermo Fisher Scientific, 35050061) with 1x GlutaMAX (1x, Thermo Fisher Scientific, 35050061), 1-Thioglycerol (0.039 uL/mL, Sigma-Aldrich, M6145), Ascorbic Acid (50ug/mL, Sigma-Aldrich, A8960), Transferrin (150ug/mL, Millipore Sigma, 10652202001), bFGF (5ng/mL, PeproTech, 100-18B), VEGF (5ng/mL, R&D Systems, 293-VE), IL-6 (10ng/mL, R&D Systems, 206-IL), IL-11 (5ng/mL, R&D Systems, 218-IL), TPO (30ng/mL, R&D Systems, 288-TPN), IGF-I (25ng/mL, R&D Systems, 291-G1), SCF (50ng/mL, R&D Systems, 255-SC), IL-3 (10ng/mL, R & D Systems, 203-IL), ROCKi/Y-27632 (10uM, Sigma-Aldrich, Y0503), BMP4 (10 ng/mL, R&D Systems, 314-BP) and Flt3L (10ng/mL, R&D Systems, 308-FK).

    Article Title: Tracking the gene expression programs and clonal relationships that underlie mast, myeloid, and T lineage specification from stem cells.
    Article Snippet: Cells were mixed approximately 30 times to achieve dissociation to a single cell suspension and TrypLE was diluted in HF buffer (Hanks’ balanced salt solution, Thermo Fisher Scientific, 14175103, + 2% FBS, Thermo Fisher Scientific, 12483020) and then cells were spun at 300xg for 5 minutes and washed in HF. .. Next the CD34-positive selection kit (Miltenyi Biotec, 130-046-702) was used to enrich for CD34+ cells whichwere cryopreserved using CryoStor CS10 (STEMCELL Technologies, 07930) following the manufacturer’s instructions. .. Endothelial to hematopoietic transition culture EHT media was prepared by combining complete StemPro34 (Thermo Fisher Scientific, 35050061) with 1x GlutaMAX (1x, Thermo Fisher Scientific, 35050061), 1-Thioglycerol (0.039 uL/mL, Sigma-Aldrich, M6145), Ascorbic Acid (50ug/mL, Sigma-Aldrich, A8960), Transferrin (150ug/mL, Millipore Sigma, 10652202001), bFGF (5ng/mL, PeproTech, 100-18B), VEGF (5ng/mL, R&D Systems, 293-VE), IL-6 (10ng/mL, R&D Systems, 206-IL), IL-11 (5ng/mL, R&D Systems, 218-IL), TPO (30ng/mL, R&D Systems, 288-TPN), IGF-I (25ng/mL, R&D Systems, 291-G1), SCF (50ng/mL, R&D Systems, 255-SC), IL-3 (10ng/mL, R & D Systems, 203-IL), ROCKi/ Y- 27632 (10uM, Sigma-Aldrich, Y0503), BMP4 (10 ng/mL, R&D Systems, 314-BP) and Flt3L (10ng/mL, R&D Systems, 308-FK).

    Article Title: The synthetic phospholipid C8-C1P determines pro-angiogenic and pro-reparative features in human macrophages restraining the proinflammatory M1-like phenotype
    Article Snippet: .. Using the CD34 positive selection kit (Miltenyi Biotec, Bergisch Gladbach, Germany), endothelial colony forming cells (ECFCs) were isolated from umbilical cord blood collected after normal full-term deliveries with the written informed consent of the mother, as described previously ( , ). ..

    Fluorescence:

    Article Title: Low input capture Hi-C (liCHi-C) identifies promoter-enhancer interactions at high-resolution
    Article Snippet: .. Hematopoietic stem and progenitor cells (HSC; CD34 + , CD38 − ) common myeloid progenitors (CMP; CD34 + , CD38 + , CD33 + ) and common lymphoid B-cell progenitors (CLP; CD34 + , CD38 + , CD19 + ) were purified by Miltenyi Biotec CD34-positive selection kit (cat. #130-046-703) and fluorescence-activated cell sorting (FACS) from four donations (two per biological replicate) of 15-to 22-week-old human fetal liver and fetal bone marrow as previously described . .. Briefly, after CD34 + selection, positive cells were stained for flow cytometry with the following fluorophore-conjugated monoclonal antibodies, all from BD Biosciences, and used at the manufacturer’s recommended concentration: CD34 PECy7 (cat. #348811; 1:20 dilution), CD38 FITC (cat. #555459; 1:5 dilution), CD19 BV421 (cat. #562440; 1:20 dilution) and CD33 APC (cat. #551378; 1:5 dilution).

    FACS:

    Article Title: Low input capture Hi-C (liCHi-C) identifies promoter-enhancer interactions at high-resolution
    Article Snippet: .. Hematopoietic stem and progenitor cells (HSC; CD34 + , CD38 − ) common myeloid progenitors (CMP; CD34 + , CD38 + , CD33 + ) and common lymphoid B-cell progenitors (CLP; CD34 + , CD38 + , CD19 + ) were purified by Miltenyi Biotec CD34-positive selection kit (cat. #130-046-703) and fluorescence-activated cell sorting (FACS) from four donations (two per biological replicate) of 15-to 22-week-old human fetal liver and fetal bone marrow as previously described . .. Briefly, after CD34 + selection, positive cells were stained for flow cytometry with the following fluorophore-conjugated monoclonal antibodies, all from BD Biosciences, and used at the manufacturer’s recommended concentration: CD34 PECy7 (cat. #348811; 1:20 dilution), CD38 FITC (cat. #555459; 1:5 dilution), CD19 BV421 (cat. #562440; 1:20 dilution) and CD33 APC (cat. #551378; 1:5 dilution).

    Sequencing:

    Article Title: Decoding human B cell ontogeny in prenatal and adult bone marrow and in vitro models via single-cell multiomics
    Article Snippet: After centrifugation, red blood cells were lysed by treating with RBC lysis buffer according to manufacturer’s instructions (eBioscience #00-4300). .. Finally, cells were counted and processed in one or more of the following way; (1) sequencing the whole population, (2) enriching for CD34-positive cells using the CD34-positive selection kit (Miltenyi Biotec #130-046-702), following manufacturer’s instructions, and sequencing, (3) enriching for CD34-positive cells and sorting for LMPP and MPP subpopulations . .. Staining antibodies: Lin-FITC: CD2 clone: RPA-2.10 (BioLegend), CD3 clone OKT3 (BioLegend), CD14 clone M5E2 (BioLegend), CD16 clone 3G8 (BioLegend), CD56 clone HCD56 (BioLegend), CD235a clone Ga-R2/HiR2 (BD Biosciences); CD34-BV786 clone 581 (BD Biosciences); CD38-BV711 clone HIT2 (BioLegend); CD90-APC-Cy7 clone 5E10 (BioLegend); CD45RA-APC clone HI100 (BioLegend), and DAPI as live death dye.

    Isolation:

    Article Title: The synthetic phospholipid C8-C1P determines pro-angiogenic and pro-reparative features in human macrophages restraining the proinflammatory M1-like phenotype.
    Article Snippet: .. Using the CD34 positive selection kit (Miltenyi Biotec, Bergisch Gladbach, Germany), endothelial colony forming cells (ECFCs) were isolated from umbilical cord blood collected after normal full-term deliveries with the written informed consent of the mother, as described previously (9, 18). ..

    Article Title: The synthetic phospholipid C8-C1P determines pro-angiogenic and pro-reparative features in human macrophages restraining the proinflammatory M1-like phenotype
    Article Snippet: .. Using the CD34 positive selection kit (Miltenyi Biotec, Bergisch Gladbach, Germany), endothelial colony forming cells (ECFCs) were isolated from umbilical cord blood collected after normal full-term deliveries with the written informed consent of the mother, as described previously ( , ). ..



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